Gabrielle Dewson1, Daniel Liefwalker1, Gabriel Cohn1, Yulong Su4, Rosalie Sears1,2,3
1Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR, USA, 2Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA, 3Brenden-Colson Center for Pancreatic Care, Oregon Health and Science University, Portland, OR, USA,4Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
In recent studies, c-Myc has been shown to localize to the nuclear pore complex (NPC) after specific post-translational modifications. The localization of c-Myc to the NPC can mediate aspects of stress response to environmental signals, and separate studies show a role for c-Myc in gene gating at the NPC. The c-MYC interacting protein partners at the NPC are still unknown and could potentially contribute to oncogenic responses governed by c-Myc. We utilized a Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins (RIME) experiment to identify proteins, which interact with C-Myc (N-262 Abcam), TPR, and other nuclear pore proteins (Mab414). The identified protein targets from the RIME experiment were then tested in mouse embryonic fibroblasts, pancreatic cancer, and lymphoma cell lines. Currently, we are examining the role of c-Myc and RIME-identified protein interactions to uncover potential therapeutic targets in PDAC.
PI/Mentor: Rosalie Sears
Co-PI/Co-Mentor: Daniel Liefwalker
Co-authors: Gabriel Cohn, Yulong Su