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Abstract

In this dissertation, the rhesus macaque embryo was evaluated for aneuploidy frequency to determine if it is a suitable translation model to study human embryology. Using single-cell DNA-Sequencing, chromosome instability was established in the rhesus macaque monkey model and found to occur in 74% of embryos at a nearly identical rate to human embryos. Additional findings of reciprocal sub-chromosomal deletions and duplications support previous reports that chromosome breakage is common in human embryos. Pairing sequencing technology with non-invasive live-cell time-lapse imaging revealed that multipolar divisions were present as well and often resulted in chaotic aneuploidy, which is defined as greater than five whole or segmental chromosomes affected in at least one blastomere where chromosome distribution amongst cells appeared unconstrained and random.

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