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Abstract

Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from the ultraviolet to the near infrared region and are optimized for conventional bandpass based fluorescence microscopy, they are not ideal for multicolor microscopy as they tend to have short Stokes shifts, restricting the number of fluorophores that can be detected in a single sample to four or five. Herein, we synthesized a library of 95 novel BODIPY (boron-dipyrromethene)-based fluorophores and screened their photophysical properties for conventional and superresolution multicolor microscopy applications. Overall, the fluorophores generated from our BODIPY-based library will enable both conventional and SRM multicolor imaging applications alone or in conjunction with commercially available fluorophores, increasing the available color palette for imaging.

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